EMEBET NIGATU BIRHANU2026-03-192024-11https://etd.hu.edu.et/handle/123456789/1334Background: The leaves of Plectranthus cylindraceus are traditionally used to treat wounds. Even though, there have been claims supporting wound healing effect, there are no scientific data on wound healing activities of the leaves of Plectranthus cylindraceus. Objective: This study aimed to evaluate wound healing activity of 80% methanol crude extract and solvent fractions of Plectranthus cylindraceus in mice. Method: The leaves of Plectranthus cylindraceus were dried, ground and macerated with 80% methanol three times successively. The crude extract was fractioned by water, ethyl acetate, and n-hexane, separately. Acute dermal toxicity tests were done by applying 2000 mg/kg of 10% (w/w) crude extract ointment. The wound healing activity of the crude extract was evaluated on excision and incision wound models. While, the fractions were evaluated on the excision wound model. The evaluation consisted of assessing the wound contraction rate, epithelialization period and histopathological analysis compared to simple ointment negative control. One-way ANOVA followed by the post-hoc Tukey test was conducted using IBM SPSS software version 27.0 for data analysis, and a p-value of <0.05 was considered statistically significant. Results: Acute dermal toxicity test result showed that topical application of 2000 mg/kg of 10%(w/w) crude extract ointment (CEO) of P.cylindraceus did show any sign of toxicity and hence considered safe. Both the 5% (w/w) and 10% (w/w) crude extract ointment exerted significant(p < 0.001) wound contraction from day 4 onwards compared to the simple ointment (SO) and resulted in reduced periods of epithelialization. The crude extract ointment treated mice exhibited a significantly increased tensile strength (p< 0.001) compared to untreated and negative control groups. The n-hexane, ethyl acetate, and aqueous fractions of the crude extract also demonstrated significant improvement in wound contraction and epithelialization time reduction on the excision wound model compared to the negative control group. The aqueous fraction demonstrated better activity compared to others. Conclusion: The positive results of the study, which include the advancement of wound contraction, reduction in the period of epithelialization, and improvement of tensile strength, validate the traditional use of Plectranthus cylindraceus for wound healing.enCrude extractfractionmethanolmicePlectranthus cylindraceuswound.Thesis