BARKILIGN SIMACHEW ANDUALEM2026-02-042024-11https://etd.hu.edu.et/handle/123456789/585This research was designed to evaluate the quality and detect adulteration from commercial honey available in the market around Debre Markos city and the surrounding areas using UV-Vis spectroscopic technique. For that, pure (real) honey samples were collected directly from the hives and commercially available honey samples were bought from the supermarket. In this study more concerned qualitative and quantitative analyzed using UV-Vis spectrometer were discriminated pure honey from adulterants honey bought from super market. For the reference 3 pure honey collected from hive were used. Calibration curve was generated by adulterating the pure honey with known concentrations of glucose syrup (0% - 30%). After that the absorbance of glucose, which is maximum at wave length of 490.56nm, was measured on the honey samples obtained from market and converted to glucose concentration using the calibration curve. The results show that the sample bought from market has more glucose concentration compared to pure honey. The order of average concentration of glucose obtained honeys collected in varies market places are as follows: 6.214% (6ADB) ˂ 20.53% (4MHR) ˂ 23.878% (3LUM) ˂ 24.417% (8DE) ˂ 25.934% (1DZ) ˂ 26.146% (2SHW) ˂ 27.016% (5MKO) ˂ 28.597% (7GW).The sample from 6ADB (6.214%) show lower glucose concentration than others and more close to pure honey. The experimental results are validated in terms of the widely used analytical parameters for method validation such as precision, linearity, limit of detection (LOD) and limit of quantification (LOQ).enhoneyadulterationBeer-lambert’s lawUV-Vis spectroscopyThesis