Browsing by Author "KIDIST BEYENE BAYOU"

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    Phytochemical Analysis of The Stem Bark Extract of Eucalyptus globules Labill
    (Hawassa University, 2024-10) KIDIST BEYENE BAYOU
    The traditional medicinal system continues to play an essential role in health care, with about 80% of the world’s inhabitants relying mainly on traditional medicines for their primary health care. The genus Eucalyptus is known to possess medicinal properties in every part of the plant. Eucalyptus globules is one of the medicinal plants that has been claimed to be used traditionally to treat several illnesses such as stomachaches, toothache, snake bite, gonorrhea, evil eyes, diarrhea, skin diseases, and headaches. The aim of this study was to investigate the phytochemicals of the stem bark of Eucalyptus globules. The stem bark was chopped into small pieces, and air dried under shade, at room temperature. The complete air-dried stem bark was pulverized into fine powder. The powdered stem bark was extracted successively with three different solvents: hexane, CHCl3:MeOH (v/v, 1:1) and methanol using cold maceration method. The percentage yield shows 0.42%, 2.4%, and 3.22% of hexane, CHCl3:MeOH (v/v, 1:1) and methanolic extracts respectively. Phytochemical screening tests of the crude extracts of theCHCl3:MeOH (v/v, 1:1) and methanolic extracts revealed the presence of the classes of secondary metabolites namely alkaloids, flavonoids, phenols, glycosides, terpenoids, saponins, and tannins. The crude n-hexane extract showed the presence of phenols, cardiac glycosides, terpenoids, steroids, and saponins. The CHCl3:MeOH (v/v, 1:1) extracts were further fractionated with various composition of hexane:ethyl acetate and ethylacetate:methanol solvent systems which resulted in three compounds,EG-1, EG-2, and EG-3. The UV-Vis and IR spectral data of the isolated compounds were generated in addition to their melting points. The structures of the isolated compounds were partially illustrated because of the absence of NMR data. The experimental UV-Vis and IR spectroscopic data were interpreted and compared with literature in order to propose the structure and the identity of the compounds. Hence the isolated compounds EG-1, EG-2 and EG-3 were proposed to be chrysin (5,7-dihydroxyflavone), purpurin (1,2,4-trihydroxy 9-10 anthraquinone) and 1,2-Dimethoxy-6-methyl-9,10-anthraquinone ,respectively.