College of Natural and Computational Sciences

Permanent URI for this communityhttps://etd.hu.edu.et/handle/123456789/89

Browse

Filter results by typing the first few letters
Now showing 1 - 1 of 1
  • No Thumbnail Available
    Item
    HABITAT CHARACTERIZATION AND INSECTICIDE RESISTANT STATUS OF ANOPHELES GAMBIAE (S.L.) IN MALARIA ENDEMIC DISTRICTS OF SIDAMA REGION, SOUTH ETHIOPIA
    (Hawassa University, 2024-05) MEDAN TAYE HUSSE
    The prevalence of malaria in Ethiopia has decreased somewhat in recent decades, but it is currently increasing as a result of the introduction of insecticide-resistant Anopheles mosquitoes. Therefore, the purpose of this study was to assess the anopheles mosquito's insecticide susceptibility status and habitat characterization in a selected study area at November. Cross sectional study was used to conduct the study. The presence of Anopheles larvae was confirmed in all water-holding habitats, and information about the habitat's perimeter, water depth, light intensity, current flow, temperature, pH, turbidity, and proximity to the closest house was noted. Additionally, information about vegetation coverage, permanence, surface debris, emergent plant, habitats and substrate type of larval holding habitats were recorded for the purpose of characterizing the habitat. Sample of anopheles larvae were taken to Akililu Lemma Institute of Pathobiology from study area in November 2024. The larvae were then reared there, their insecticide susceptibility tested, and their species was identified using the Coetzee 2020 identification key. In order to test for insecticide susceptibility, 100 adult Anopheles mosquitoes were exposed to each insecticide that the World Health Organization (WHO) recommended (propoxur 0.1%, deltamethrin 0.05%, and alpha-cypermethrin 0.45%). The knockdown times (KDT), percentage knockdown, and percentage mortalities of the exposed mosquitoes were noted. Anopheles gambiae s.l., the species of mosquito that was identified, was resistant to propoxur (0.1%) and delthametrin (0.05%), but it was completely susceptible to alpha cypermethrin (0.45%) across all study sites. In all research sites, alpha-cypermethrin insecticide took less time (18.67-24.23min) to kill 50% of Anopheles gambiae s.l. In every study area, the KDT50 of Anopheles gambiae exposed to propoxur insecticide was high, ranging from 51.95 to 56.25 minutes. Anopheles mosquito species that dominate study area was Anopheles gambiae s.l; it was susceptible to Alpha cypermethrin and resistant to delthametrin and propoxur and it prefer to breed in habitat which exposed to sun light, had stone substrate and had no canopy cover. Anopheles gambiae (s.l) that inhabit Chuko, Dale and Loka Abaya was susceptible to Alphacypermethrin and resistant to Deltamethring & Propoxure. This species was highly productive in habitat like ditches, burrow pits and road puddle which exposed to sun light, without canopy cover and with stony substrate.